Basic Vector Information
- Vector Name:
- pMSCVhyg
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6945 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Copy Number:
- High copy number
- Promoter:
- MSCV
pMSCVhyg vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMSCVhyg vector Sequence
LOCUS pMSCVhyg. 6945 bp DNA circular SYN 01-JAN-1980 DEFINITION Retroviral vector with a hygromycin resistance marker optimized for gene expression in hematopoietic, embryonic stem, or embryonic carcinoma cells. ACCESSION . VERSION . KEYWORDS pMSCVhyg. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6945) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 6945) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6945 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 1..515 /label=MSCV /note="Murine embryonic stem cell virus promoter including the enhancer and promoter region of PCMV virus and 5' untranslated region of dl-587rev retrovirus" misc_feature 579..920 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS 987..1403 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" misc_feature 1410..1433 /label=MCS /note="MCS" /note="multiple cloning site" promoter 1438..1937 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 1955..2977 /label=HygR /note="aminoglycoside phosphotransferase from E. coli" LTR 3338..3852 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" protein_bind complement(4014..4030) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4038..4068) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4083..4104) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4392..4980) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5154..6011) /label=AmpR /note="beta-lactamase" promoter complement(6012..6116) /label=AmpR promoter LTR 6944..6945 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus"
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