Basic Vector Information
- Vector Name:
- pMSCVpuro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6264 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Copy Number:
- High copy number
- Promoter:
- MSCV
pMSCVpuro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMSCVpuro vector Sequence
LOCUS pMSCVpuro. 6264 bp DNA circular SYN 01-JAN-1980
DEFINITION Retroviral vector with a puromycin resistance marker optimized for
gene expression in hematopoietic, embryonic stem, or embryonic
carcinoma cells.
ACCESSION .
VERSION .
KEYWORDS pMSCVpuro.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6264)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6264)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6264
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..515
/label=MSCV
/note="Murine embryonic stem cell virus promoter including
the enhancer and promoter region of PCMV virus and 5'
untranslated region of dl-587rev retrovirus"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 1410..1433
/label=MCS
/note="MCS"
/note="multiple cloning site"
promoter 1438..1937
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 1958..2554
/label=PuroR
/note="puromycin N-acetyltransferase"
LTR 2657..3171
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind complement(3333..3349)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3357..3387)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3402..3423)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3711..4299)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4473..5330)
/label=AmpR
/note="beta-lactamase"
promoter complement(5331..5435)
/label=AmpR promoter
LTR 6263..6264
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
This page is informational only.