pRetro-Lib vector (V010394)

LOCUS       pRetro-Lib.        4891 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral vector for delivery and expression of libraries or 
            individual genes.
ACCESSION   .
VERSION     .
KEYWORDS    pRetro-Lib
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..589
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1470..1502
                     /label=MCS A
                     /note="MCS A"
                     /note="multiple cloning site"
     polyA_signal    1565..1699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1695..1723
                     /label=MCS B
                     /note="MCS B"
                     /note="multiple cloning site"
     LTR             1763..2356
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    2567..2707
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2893..3481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3655..4512)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4513..4617)
                     /label=AmpR promoter