ptTS-Neo vector (V010350)

Basic Vector Information

      • Vector Name:
      • ptTS-Neo
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6365 bp
      • Type:
      • Viral Expression & Packaging Vectors
      • Source/Author:
      • Clontech
      • Selection Marker:
      • Neomycin/G418(Geneticin)
      • Copy Number:
      • High copy number

ptTS-Neo vector Vector Map

ptTS-Neo6365 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300SV40 promoterNeoR/KanRHSV TK poly(A) signalCMV enhancerCMV promotertTSbeta-globin intronHBBbeta-globin poly(A) signaloriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

ptTS-Neo vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       ptTS-Neo.        6365 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing tTS, which represses expression from 
            Tet-responsive promoters in the absence of doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    ptTS-Neo.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..246
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             289..1080
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1315..1363
                     /label=HSV TK poly(A) signal
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     enhancer        1449..1828
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1829..2032
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2135..2980
                     /label=tTS
                     /note="fusion of a hybrid tetracycline repressor TetR with
                     a nuclear localization signal and a KRAB silencing domain"
     intron          3004..3576
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             3580..3702
                     /gene="HBB"
                     /label=HBB
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    3773..3828
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(4546..5134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5308..6165)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6166..6270)
                     /label=AmpR promoter

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