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ptTS-Neo vector (V010350)

Basic Vector Information

Vector Name:
ptTS-Neo
Antibiotic Resistance:
Ampicillin
Length:
6365 bp
Type:
Viral Expression & Packaging Vectors
Replication origin:
ori
Source/Author:
Clontech
Selection Marker:
Neomycin/G418(Geneticin)
Copy Number:
High copy number
Promoter:
SV40

ptTS-Neo vector Map

Copy Sequence View Map
ptTS-Neo6365 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300SV40 promoterNeoR/KanRHSV TK poly(A) signalCMV enhancerCMV promotertTSbeta-globin intronHemoglobin subunit betabeta-globin poly(A) signaloriAmpRAmpR promoter

ptTS-Neo vector Sequence

LOCUS       V010350                 6365 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010350
VERSION     V010350
KEYWORDS    ptTS-Neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..246
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             289..1080
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1315..1363
                     /label="HSV TK poly(A) signal"
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     enhancer        1449..1828
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1829..2032
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2135..2980
                     /label="tTS"
                     /note="fusion of a hybrid tetracycline repressor TetR with
                     a nuclear localization signal and a KRAB silencing domain"
     intron          3004..3576
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             3580..3702
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    3773..3828
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(4546..5134)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5308..6165)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6166..6270)
                     /label="AmpR promoter"

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