Basic Vector Information
ptTS-Neo vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
ptTS-Neo vector Sequence
LOCUS ptTS-Neo. 6365 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for expressing tTS, which represses expression from Tet-responsive promoters in the absence of doxycycline. ACCESSION . VERSION . KEYWORDS ptTS-Neo. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6365) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 6365) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6365 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 36..246 /label=SV40 promoter /note="SV40 early promoter" CDS 289..1080 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 1315..1363 /label=HSV TK poly(A) signal /note="HSV TK poly(A) signal" /note="herpesvirus thymidine kinase polyadenylation signal" enhancer 1449..1828 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1829..2032 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 2135..2980 /label=tTS /note="fusion of a hybrid tetracycline repressor TetR with a nuclear localization signal and a KRAB silencing domain" intron 3004..3576 /label=beta-globin intron /note="intron from rabbit beta-globin gene" CDS 3580..3702 /gene="HBB" /label=HBB /note="Hemoglobin subunit beta from Colobus guereza. Accession#: Q9TT33" polyA_signal 3773..3828 /label=beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" rep_origin complement(4546..5134) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5308..6165) /label=AmpR /note="beta-lactamase" promoter complement(6166..6270) /label=AmpR promoter
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