pFA6-kanMX4 vector (V010318)

LOCUS       40924_19166        3941 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid carrying the kanMX selector module conferring kanamycin 
            resistance. Also known as pFA6a-kanMX4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Wach A, Brachat A, Pohlmann R, Philippsen P.
  TITLE     New heterologous modules for classical or PCR-based gene disruptions
            in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1994;10:1793-808.
  PUBMED    7747518
REFERENCE   2  (bases 1 to 3941)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3941)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1994"; volume: "10"; pages: "1793-808"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..1471
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(1579..1597)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1855..2443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2617..3474)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3475..3579)
                     /label=AmpR promoter
     promoter        3925..3941
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"