pGAPZα C vector (V010271)

LOCUS       pGAPZ-C.        3152 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for constitutive expression of a secreted 
            protein. For other reading frames, use pGAPZ-alpha A or pGAPZ-alpha 
            B.
ACCESSION   .
VERSION     .
KEYWORDS    pGAPZ-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3152)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize within the GAP promoter for integration.
FEATURES             Location/Qualifiers
     source          1..3152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..483
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             493..759
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    759..833
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             832..861
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             877..894
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      973..1219
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1234..1645
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1652..1699
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1718..2089
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2158..2405
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2480..3068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"