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pPIC6 B vector (V010251)

Basic Vector Information

Vector Name:
pPIC6 B
Antibiotic Resistance:
Blasticidin
Length:
3380 bp
Type:
Yeast Plasmids
Replication origin:
ori
Source/Author:
Invitrogen (Life Technologies)
Copy Number:
High copy number
Promoter:
AOX1

pPIC6 B vector Map

Copy Sequence View Map
pPIC6 B3380 bp6001200180024003000AOX1 promoterMyc6xHisAOX1 terminatorTEF1 promoterEM7 promoterBSDCYC1 terminatorori

pPIC6 B vector Sequence

LOCUS       40924_34515        3380 bp DNA     circular SYN 13-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3380)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             1010..1039
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1055..1072
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1152..1398
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1413..1824
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1832..1879
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1898..2293
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     terminator      2390..2637
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2712..3300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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