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pPIC6α B vector (V010248)

Basic Vector Information

Vector Name:
pPIC6α B
Antibiotic Resistance:
Blasticidin
Length:
3650 bp
Type:
Yeast Plasmids
Replication origin:
ori
Source/Author:
Invitrogen (Life Technologies)
Copy Number:
High copy number
Promoter:
AOX1

pPIC6α B vector Vector Map

pPIC6α B3650 bp60012001800240030003600AOX1 promoteralpha-factor secretion signalMyc6xHisAOX1 terminatorTEF1 promoterEM7 promoterBSDCYC1 terminatorori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPIC6α B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pPIC6-B.        3650 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein. For other reading frames, use pPIC6-alpha A or 
            pPIC6-alpha C.
ACCESSION   .
VERSION     .
KEYWORDS    pPIC6-B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3650)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3650)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    1206..1280
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             1279..1308
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1324..1341
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1421..1667
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1682..2095
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2102..2149
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2168..2563
                     /label=BSD
                     /note="blasticidin S deaminase"
     terminator      2660..2907
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2982..3570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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