YCplac33 vector (V010192)

Basic Vector Information

      • Vector Name:
      • YCplac33
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5603 bp
      • Type:
      • Yeast Plasmids
      • Source/Author:
      • Gietz RD, Sugino A.
      • Copy Number:
      • High copy number
      • 5' Primer:
      • M13 fwd
      • 3' Primer:
      • M13 rev

YCplac33 vector Vector Map

YCplac335603 bp60012001800240030003600420048005400lac promoterlac operatorM13 revCEN/ARSURA3URA3 promoterAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YCplac33 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5603 bp ds-DNA    circular SYN 29-11-2013
DEFINITION  Yeast centromeric plasmid with a URA3 marker.
KEYWORDS    YCplac33
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Gietz RD, Sugino A.
  TITLE     New yeast-Escherichia coli shuttle vectors constructed with in vitro
            mutagenized yeast genes lacking six-base pair restriction sites.
  JOURNAL   Gene 1988;74:527-34.
  PUBMED    3073106
REFERENCE   2  (bases 1 to 5603)
  TITLE     Direct Submission
COMMENT     The sequence of this plasmid was reconstructed using the description
            from Gietz and Sugino.
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        142..172
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
     CDS             216..554
                     /product="LacZ-alpha fragment of beta-galactosidase"
     misc_feature    233..289
                     /note="pUC19 multiple cloning site"
     primer_bind     complement(290..306)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
     misc_feature    1064..2227
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     CDS             complement(2562..3365)
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
     promoter        complement(3366..3586)
                     /gene="S. cerevisiae URA3"
                     /note="URA3 promoter"
     promoter        3697..3801
                     /note="AmpR promoter"
     CDS             3802..4662
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      4833..5421
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 

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