NovoPro

YIp351 vector

Basic Vector Information

Basic Vector Information
Vector Name YIp351 Antibiotic Resistance Ampicillin
Length 4406 bp Type Yeast Plasmids
Source Hill JE, Myers AM, Koerner TJ, Tzagoloff A. Copy Number High copy number
5' Primer M13 fwd 3' Primer M13 rev

YIp351 vector Vector Map

YIp351 vector Vector Map

YIp351 vector Sequence

LOCUS       YIp351        4406 bp    DNA     circular
DEFINITION  Yeast/E. coli integrating shuttle vector with a LEU2 marker.
ACCESSION   L14756
KEYWORDS    YIp351.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Hill JE, Myers AM, Koerner TJ, Tzagoloff A.
  TITLE     Yeast/E. coli shuttle vectors with multiple unique restriction 
            sites.
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   .
  TITLE     Direct Submission
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /vntifkey="98"
     CDS             1..303
                     /codon_start=1
                     /gene="lacZ"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /vntifkey="4"
                     /label=lacZ
                     /note="lacZ-alpha"
     misc_feature    15..71
                     /gene="
                     "
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="MCS pUC19 multiple cloning site"
     primer_bind     complement(75..91)
                     /vntifkey="28"
                     /label=Primer_Bind_1
                     /note="M13 fwd common sequencing primer, one of multiple similar  variants"
     CDS             complement(759..1853)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /vntifkey="4"
                     /label=S.\cerevisiae\LEU2
                     /note="LEU2 yeast auxotrophic marker"
     promoter        complement(1854..2258)
                     /gene="S. cerevisiae LEU2"
                     /vntifkey="30"
                     /label=S.\cerevisiae\LEU2
                     /note="LEU2 promoter"
     promoter        2285..2389
                     /gene="bla"
                     /vntifkey="30"
                     /label=bla
                     /note="AmpR promoter"
     CDS             2390..3250
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=bla
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     rep_origin      3421..4009
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ori high-copy-number colE1/pMB1/pBR322/pUC origin of  replication"
     promoter        4333..4363
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="lac promoter promoter for the E. coli lac operon"
     protein_bind    4371..4387
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=Protein_Bind_1
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4395..5
                     /vntifkey="28"
                     /label=Primer_Bind_2
                     /note="M13 rev common sequencing primer, one of multiple similar  variants"

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