NovoPro

YIp352 vector

Basic Vector Information

Basic Vector Information
Vector Name YIp352 Antibiotic Resistance Ampicillin
Length 4303 bp Type Yeast Plasmids
Source Hill JE, Myers AM, Koerner TJ, Tzagoloff A. Copy Number High copy number
5' Primer M13 fwd 3' Primer M13 rev

YIp352 vector Vector Map

YIp352 vector Vector Map

YIp352 vector Sequence

LOCUS       YIp352        4303 bp    DNA     circular
DEFINITION  Yeast/E. coli integrating shuttle vector with a URA3 marker.
ACCESSION   L14759
KEYWORDS    YIp352.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Hill JE, Myers AM, Koerner TJ, Tzagoloff A.
  TITLE     Yeast/E. coli shuttle vectors with multiple unique restriction 
            sites.
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   .
  TITLE     Direct Submission
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Shuttle vector YIp352"
                     /focus
                     /mol_type="genomic DNA"
                     /db_xref="taxon:118282"
                     /vntifkey="98"
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /vntifkey="98"
     source          286..1610
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4932"
                     /vntifkey="98"
     source          1611..4303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /vntifkey="98"
     CDS             1..291
                     /codon_start=1
                     /gene="lacZ"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /vntifkey="4"
                     /label=lacZ
                     /note="lacZ-alpha"
     misc_feature    15..71
                     /gene="
                     "
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="MCS pUC19 multiple cloning site"
     primer_bind     complement(75..91)
                     /vntifkey="28"
                     /label=Primer_Bind_1
                     /note="M13 fwd common sequencing primer, one of multiple similar  variants"
     CDS             complement(364..1167)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required
                     for uracil biosynthesis"
                     /vntifkey="4"
                     /label=S.\cerevisiae\URA3
                     /note="URA3 yeast auxotrophic marker, counterselectable with  5-fluoroorotic acid (5-FOA)"
     promoter        complement(1168..1378)
                     /gene="S. cerevisiae URA3"
                     /vntifkey="30"
                     /label=S.\cerevisiae\URA3
                     /note="URA3 promoter"
     promoter        2182..2286
                     /gene="bla"
                     /vntifkey="30"
                     /label=bla
                     /note="AmpR promoter"
     CDS             2287..3147
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=bla
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     rep_origin      3318..3906
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ori high-copy-number colE1/pMB1/pBR322/pUC origin of  replication"
     promoter        4230..4260
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="lac promoter promoter for the E. coli lac operon"
     protein_bind    4268..4284
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=Protein_Bind_1
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4292..5
                     /vntifkey="28"
                     /label=Primer_Bind_2
                     /note="M13 rev common sequencing primer, one of multiple similar  variants"

This page is informational only.