YIplac128 vector

Basic Vector Information

Basic Vector Information
Vector Name YIplac128 Antibiotic Resistance Ampicillin
Length 4293 bp Type Yeast Plasmids
Source Gietz RD, Sugino A. Copy Number High copy number
5' Primer M13 fwd 3' Primer M13 rev

YIplac128 vector Vector Map

YIplac128 vector Vector Map

YIplac128 vector Sequence

LOCUS       YIplac128        4293 bp    DNA     circular
DEFINITION  Yeast integrating plasmid with a LEU2 marker.
KEYWORDS    YIplac128.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Gietz RD, Sugino A.
  TITLE     New yeast-Escherichia coli shuttle vectors constructed with in vitro
            mutagenized yeast genes lacking six-base pair restriction sites.
REFERENCE   2  (bases 1 to 4293)
  TITLE     Direct Submission
            from Gietz and Sugino.
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        142..172
                     /note="lac promoter promoter for the E. coli lac operon"
     protein_bind    180..196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /note="M13 rev common sequencing primer, one of multiple similar  variants"
     CDS             216..539
                     /product="LacZ-alpha fragment of beta-galactosidase"
     misc_feature    233..289
                     /note="MCS pUC19 multiple cloning site"
     primer_bind     complement(290..306)
                     /note="M13 fwd common sequencing primer, one of multiple similar  variants"
     CDS             complement(797..1891)
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /note="LEU2 yeast auxotrophic marker"
     promoter        complement(1892..2299)
                     /gene="S. cerevisiae LEU2"
                     /note="LEU2 promoter"
     promoter        2387..2491
                     /note="AmpR promoter"
     CDS             2492..3352
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     rep_origin      3523..4111
                     /note="ori high-copy-number colE1/pMB1/pBR322/pUC origin of  replication"

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