YIplac204 vector

Basic Vector Information

Basic Vector Information
Vector Name YIplac204 Antibiotic Resistance Ampicillin
Length 3545 bp Type Yeast Plasmids
Source Gietz RD, Sugino A. Copy Number High copy number
5' Primer M13 fwd 3' Primer M13 rev

YIplac204 vector Vector Map

YIplac204 vector Vector Map

YIplac204 vector Sequence

LOCUS       YIplac204        3545 bp    DNA     circular
DEFINITION  Yeast integrating plasmid with a TRP1 marker.
KEYWORDS    YIplac204.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Gietz RD, Sugino A.
  TITLE     New yeast-Escherichia coli shuttle vectors constructed with in vitro
            mutagenized yeast genes lacking six-base pair restriction sites.
REFERENCE   2  (bases 1 to 3545)
  TITLE     Direct Submission
            from Gietz and Sugino.
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        142..172
                     /note="lac promoter promoter for the E. coli lac operon"
     protein_bind    180..196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /note="M13 rev common sequencing primer, one of multiple similar  variants"
     CDS             216..539
                     /product="LacZ-alpha fragment of beta-galactosidase"
     misc_feature    233..289
                     /note="MCS pUC19 multiple cloning site"
     primer_bind     complement(290..306)
                     /note="M13 fwd common sequencing primer, one of multiple similar  variants"
     rep_origin      696..936
                     /note="ARS1 S. cerevisiae autonomously replicating sequence  ARS1/ARS416"
     CDS             complement(775..1449)
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required
                     for tryptophan biosynthesis"
                     /note="TRP1 yeast auxotrophic marker"
     promoter        complement(1450..1551)
                     /gene="S. cerevisiae TRP1"
                     /note="TRP1 promoter"
     promoter        1639..1743
                     /note="AmpR promoter"
     CDS             1744..2604
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     rep_origin      2775..3363
                     /note="ori high-copy-number colE1/pMB1/pBR322/pUC origin of  replication"

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