YIplac211 vector

Basic Vector Information

Basic Vector Information
Vector Name YIplac211 Antibiotic Resistance Ampicillin
Length 3797 bp Type Yeast Plasmids
Source Gietz RD, Sugino A. Copy Number High copy number
5' Primer M13 fwd 3' Primer M13 rev

YIplac211 vector Vector Map

YIplac211 vector Vector Map

YIplac211 vector Sequence

LOCUS       YIplac211        3797 bp    DNA     circular
DEFINITION  Yeast integrating plasmid with a URA3 marker.
KEYWORDS    YIplac211.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Gietz RD, Sugino A.
  TITLE     New yeast-Escherichia coli shuttle vectors constructed with in vitro
            mutagenized yeast genes lacking six-base pair restriction sites.
REFERENCE   2  (bases 1 to 3797)
  TITLE     Direct Submission
            from Gietz and Sugino.
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        142..172
                     /note="lac promoter promoter for the E. coli lac operon"
     protein_bind    180..196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /note="M13 rev common sequencing primer, one of multiple similar  variants"
     CDS             216..539
                     /product="LacZ-alpha fragment of beta-galactosidase"
     misc_feature    233..289
                     /note="MCS pUC19 multiple cloning site"
     primer_bind     complement(290..306)
                     /note="M13 fwd common sequencing primer, one of multiple similar  variants"
     CDS             complement(774..1577)
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required
                     for uracil biosynthesis"
                     /note="URA3 yeast auxotrophic marker, counterselectable with  5-fluoroorotic acid (5-FOA)"
     promoter        complement(1578..1798)
                     /gene="S. cerevisiae URA3"
                     /note="URA3 promoter"
     promoter        1891..1995
                     /note="AmpR promoter"
     CDS             1996..2856
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     rep_origin      3027..3615
                     /note="ori high-copy-number colE1/pMB1/pBR322/pUC origin of  replication"

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