NovoPro

YRp7 vector

Basic Vector Information

Basic Vector Information
Vector Name YRp7 Antibiotic Resistance Ampicillin
Length 5817 bp Type Yeast Plasmids
Source Struhl K, Stinchcomb DT, Scherer S, Davis RW. Copy Number High copy number
Promoter TRP1

YRp7 vector Vector Map

YRp7 vector Vector Map

YRp7 vector Sequence

LOCUS       YRp7        5817 bp    DNA     circular
DEFINITION  Yeast replicating vector with a TRP1 marker.
ACCESSION   U03501
KEYWORDS    YRp7.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Struhl K, Stinchcomb DT, Scherer S, Davis RW.
  TITLE     High-frequency transformation of yeast: autonomous replication of 
            hybrid DNA molecules.
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   .
  TITLE     Direct Submission
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Cloning vector YRp7"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="unassigned DNA"
                     /db_xref="taxon:31782"
                     /vntifkey="98"
     rep_origin      7..844
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ARS1 S. cerevisiae autonomously replicating sequence  ARS1/ARS416"
     CDS             complement(683..1357)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required
                     for tryptophan biosynthesis"
                     /vntifkey="4"
                     /label=S.\cerevisiae\TRP1
                     /note="TRP1 yeast auxotrophic marker"
     promoter        complement(1358..1459)
                     /gene="S. cerevisiae TRP1"
                     /vntifkey="30"
                     /label=S.\cerevisiae\TRP1
                     /note="TRP1 promoter"
     promoter        1467..1495
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="tet promoter E. coli promoter for tetracycline efflux protein  gene"
     CDS             1543..2733
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /vntifkey="4"
                     /label=tet
                     /note="TcR confers resistance to tetracycline"
     CDS             3374..3565
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /vntifkey="4"
                     /label=rop
                     /note="rop"
     rep_origin      complement(3993..4581)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="ori high-copy-number colE1/pMB1/pBR322/pUC origin of  replication"
     CDS             complement(4752..5612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=bla
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(5613..5717)
                     /gene="bla"
                     /vntifkey="30"
                     /label=bla
                     /note="AmpR promoter"

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