Basic Vector Information
- Vector Name:
- YRp7
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5817 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Source/Author:
- Struhl K, Stinchcomb DT, Scherer S, Davis RW.
- Copy Number:
- High copy number
- Promoter:
- TRP1
YRp7 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YRp7 vector Sequence
LOCUS 40924_49792 5817 bp DNA circular SYN 17-DEC-2018
DEFINITION Yeast replicating vector YRp7, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5817)
AUTHORS Struhl K, Stinchcomb DT, Scherer S, Davis RW.
TITLE High-frequency transformation of yeast: autonomous replication of
hybrid DNA molecules
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 76 (3), 1035-1039 (1979)
PUBMED 375221
REFERENCE 2 (bases 1 to 5817)
AUTHORS Stillman DJ.
TITLE Direct Submission
JOURNAL Submitted (16-NOV-1993) David J. Stillman, Dept. of Cellular, Viral
and Molecular Biology, University of Utah Medical Center, Salt Lake
City, UT 84132 USA
REFERENCE 3 (bases 1 to 5817)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5817)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
Acad. Sci. U.S.A."; date: "1979"; volume: "76"; issue: "3"; pages:
"1035-1039"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-NOV-1993) David J. Stillman, Dept. of Cellular, Viral and
Molecular Biology, University of Utah Medical Center, Salt Lake
City, UT 84132 USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5817
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(7..844)
/direction=LEFT
/label=ARS1
/note="S. cerevisiae autonomously replicating sequence
ARS1/ARS416"
promoter complement(1358..1459)
/label=TRP1 promoter
promoter 1467..1495
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 1543..2730
/codon_start=1
/label=TcR
/note="tetracycline efflux protein"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
CDS 3374..3562
/codon_start=1
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
/translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 3667..3807
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(3993..4581)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4755..5612)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5613..5717)
/label=AmpR promoter
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