Basic Vector Information
- Vector Name:
- pHUE
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5942 bp
- Type:
- Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- Catanzariti,A.M., Soboleva,T.A., Jans,D.A., Board,P.G. and Baker,R.T.
- Copy Number:
- High copy number
- Promoter:
- T7
- Cloning Method:
- Enzyme Cut
- 5' Primer:
- T7 promoter
- 3' Primer:
- T7 terminator
- Fusion Tag:
- UBA52
- Expression Method:
- IPTG induced
pHUE vector Map
pHUE vector Sequence
LOCUS pHUE. 5942 bp DNA circular SYN 28-NOV-2018
DEFINITION Bacterial vector for inducible expression of N-terminally
6xHis-tagged proteins.
ACCESSION .
VERSION .
KEYWORDS pHUE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5942)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5942)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5942
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 156..174
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 175..199
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 214..236
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 243..245
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 255..272
/label=6xHis
/note="6xHis affinity tag"
CDS 282..299
/label=thrombin site
/note="thrombin recognition and cleavage site"
CDS 303..530
/label=ubiquitin
/note="human ubiquitin C"
misc_feature 528..577
/label=MCS
/note="MCS"
/note="multiple cloning site"
terminator 636..683
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 972..1076
/label=AmpR promoter
CDS 1077..1934
/label=AmpR
/note="beta-lactamase"
rep_origin 2108..2696
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2882..3024)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3129..3317)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(4595..4616)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(4632..5711)
/label=lacI
/note="lac repressor"
promoter complement(5712..5789)
/label=lacI promoter
This page is informational only.