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Product Name
Recombinant ZFP91 protein (His tag)
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Description
Introducing the recombinant E3 ubiquitin-protein ligase ZFP91 fragment (residues 309-466), a stable and soluble protein unit designed for structural and biochemical studies. This fragment supports research into the non-canonical NF-kappa-B2 pathway and cell proliferation mechanisms, offering a reliable tool for life science investigations.
E3 ubiquitin-protein ligase ZFP91 (EC 2.3.2.27) is an atypical RING-type E3 ubiquitin transferase that plays a critical role in the non-canonical NF-kappa-B2/NFKB2 pathway. It mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilization and activation of MAP3K14/NIK, and may also contribute to cell proliferation and anti-apoptotic processes. The full-length protein is localized in the nucleus, as supported by experimental evidence. This recombinant fragment (309-466) was selected to provide a stable and soluble region for detailed analysis of ZFP91's functional mechanisms.
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Protein name
E3 ubiquitin-protein ligase ZFP91
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Uniprot ID
Q96JP5
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Gene Name
ZFP91; ZNF757
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 309-466 aa (Q96JP5) were fused with N-terminal 6His tag.
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Sequence
MSKIKHHHHH HGGSQYVRCE MEGCGTVLAH PRYLQHHIKY QHLLKKKYVC PHPSCGRLFR LQKQLLRHAK HHTDQRDYIC EYCARAFKSS HNLAVHRMIH TGEKPLQCEI CGFTCRQKAS LNWHMKKHDA DSFYQFSCNI CGKKFEKKDS VVAHKAKSHP EVLIAEALAA NA
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 19.98 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 20 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant ZFP91 protein (His tag) SDS-PAGE
M: Marker; Lane 1: ZFP91; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"