Z-VAD-FMK peptide

Z-VAD-FMK peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Z-VAD-FMK, Benzyloxycarbonyl (Z) at N terminal, Fluoromethylketone (FMK) at C terminal

Benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone

3

95.25%

C₂₂H₃₀FN₃O₇

467.49

Synthetic

Powder

Z-VAD-FMK (Z-Val-Ala-Asp(OMe)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor. Z-VAD-FMK inhibits caspase processing and apoptosis induction in tumour cells in vitro with an IC50 of 0.0015 - 5.8 mM). Z-VAD-FMK is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability. Z-VAD-FMK is active in vivo and can inhibit apoptosis in numerous settings.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Slee et al (1996) Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32. Biochem.J. 315 21 PMID: 8670109
• Ilangovan et al (2003) Inhibition of apoptosis by Z-VAD-fmk in SMN-depleted S2 cells. J. Biol. Chem. 278(33) 30993 PMID: 12783893
• Simmons et al (2009) Pretreatment with pancaspase inhibitor (Z-VAD-FMK) delays but does not prevent intraperitoneal heat-killed group B Streptococcus-induced preterm delivery in a pregnant mouse model. Infect. Dis. Obstet. Gynecol. 2009 749432 PMID: 20069051

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.