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  • Z-IETD-AFC peptide

Z-IETD-AFC peptide

Not For Human Use, Lab Use Only.

Cat.#: 319647

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* Buy 1 get 1 free (?) What is TFA?

Special Price 249.00 USD

Availability: 1-2 weeks
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Product Information

  • Product Name
    Z-IETD-AFC peptide
  • Documents

    Batch to batch variation of the purity

  • Sequence Shortening
    Cbz-IETD-AFC
  • Sequence
    Cbz-Ile-Glu-Thr-Asp-AFC
  • Length (aa)
    4
  • Peptide Purity (HPLC)
    95.63%
  • Molecular Formula
    C37H42F3N5O13
  • Molecular Weight
    821.75
  • CAS No.
    219138-02-0
  • PubChem CID
    71312079
  • Source
    Synthetic
  • Form
    Powder
  • Description
    Z-IETD-AFC is a fluorogenic substrate used to assess the activity of caspase 8 and Granzyme B. Upon enzymatic cleavage by caspase-8 or granzyme B, 7-amino-4-trifluoromethylcoumarin (AFC) is released and its fluorescence can be used to quantify caspase-8 or granzyme B activity. AFC displays excitation/emission maxima of 400/505 nm, respectively.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Nie Z, Phenix BN, Lum JJ, Alam A, Lynch DH, Beckett B, Krammer PH, Sekaly RP, Badley AD. HIV-1 protease processes procaspase 8 to cause mitochondrial release of cytochrome c, caspase cleavage and nuclear fragmentation. Cell Death Differ. 2002 Nov;9(11):1172-84. doi: 10.1038/sj.cdd.4401094. PMID: 12404116.
  • About TFA salt

    Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

    Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

    Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

    In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"