Z-GPR-AMC peptide

Z-GPR-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Cbz-GPR-AMC

Cbz-Gly-Pro-Arg-AMC

3

95.28%

C₃₁H₃₇N₇O₇

619.67

Synthetic

Powder

The peptide Z-GPR-AMC (benzyloxycarbonyl-glycine-proline-arginine-7-amino-4-methylcoumarin) is a fluorogenic substrate commonly used to measure the proteolytic activity of enzymes, particularly cathepsin K. Upon enzymatic cleavage, the 7-amino-4-methylcoumarin (AMC) is released, which fluoresces and allows quantification of enzymatic activity. This substrate is especially useful in studies involving cysteine proteases like cathepsins, and it has been applied in the study of diseases such as osteoporosis, cancer metastasis, and atherosclerosis. Z-GPR-AMC is considered specific for cathepsin K but can also be hydrolyzed to some extent by other cathepsins, such as cathepsin B. Its use is often paired with inhibitors like CA-074 to prevent cross-reactivity with cathepsin B, ensuring more accurate measurements of cathepsin K activity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Li WA, Barry ZT, Cohen JD, Wilder CL, Deeds RJ, Keegan PM, Platt MO. Detection of femtomole quantities of mature cathepsin K with zymography. Anal Biochem. 2010 Jun 1;401(1):91-8. doi: 10.1016/j.ab.2010.02.035. Epub 2010 Mar 3. PMID: 20206119.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.