Z-Arg-Arg-AMC peptide

Z-Arg-Arg-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Cbz-RR-AMC

Cbz-Arg-Arg-AMC

2

95.85%

C₃₀H₃₉N₉O₆

621.68

136132-67-7

102601636

Synthetic

Powder

The peptide Z-Arg-Arg-AMC is a highly selective substrate used to measure cathepsin B activity. It is utilized in an assay to survey cancer cell lines for cathepsin B activity. The use of the dibasic substrate Z-Arg-Arg-AMC is preferred over the monobasic substrate Z-Phe-Arg-AMC because there are other species of cysteine proteases within the cells which can hydrolyze Z-Phe-Arg-AMC (e.g. cathepsin L) and thus can contribute to the proteolytic activity measured by this substrate.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Hulkower KI, Butler CC, Linebaugh BE, Klaus JL, Keppler D, Giranda VL, Sloane BF. Fluorescent microplate assay for cancer cell-associated cathepsin B. Eur J Biochem. 2000 Jul;267(13):4165-70. doi: 10.1046/j.1432-1327.2000.01458.x. PMID: 10866820.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.