WT1 126-134 (HLA-A*02:01) peptide

WT1 126-134 (HLA-A*02:01) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RMFPNAPYL-OH

H-Arg-Met-Phe-Pro-Asn-Ala-Pro-Tyr-Leu-OH

9

99.67%

C₅₂H₇₇N₁₃O₁₂S

1108.31

Synthetic

Powder

RMFPNAPYL is a linear peptidic epitope (epitope ID54882) studied as part of Wilms tumor protein from Homo sapiens (human). This epitope has been studied for immune reactivity, tested in T cell assays and MHC ligand assays WT-1 126-134 peptide RMFPNAPYL (HLA-A*02:01) for stimulation of T cells. Single peptide (RMFPNAPYL) for stimulation of human WT 1-specific CD8+ T cells.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Augsberger C, Hänel G, Xu W, Pulko V, Hanisch LJ, Augustin A, Challier J, Hunt K, Vick B, Rovatti PE, Krupka C, Rothe M, Schönle A, Sam J, Lezan E, Ducret A, Ortiz-Franyuti D, Walz AC, Benz J, Bujotzek A, Lichtenegger FS, Gassner C, Carpy A, Lyamichev V, Patel J, Konstandin N, Tunger A, Schmitz M, von Bergwelt-Baildon M, Spiekermann K, Vago L, Jeremias I, Marrer-Berger E, Umaña P, Klein C, Subklewe M. Targeting intracellular WT1 in AML with a novel RMF-peptide-MHC-specific T-cell bispecific antibody. Blood. 2021 Dec 23;138(25):2655-2669. doi: 10.1182/blood.2020010477. Erratum in: Blood. 2022 Mar 31;139(13):2086-2087. PMID: 34280257; PMCID: PMC9037755.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.