WRW4 peptide

WRW4 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-WRWWWW-NH2

H-Trp-Arg-Trp-Trp-Trp-Trp-NH2

6

95.85%

C₆₁H₆₅N₁₅O₆

1104.26

Synthetic

Powder

WRW4 is a selective antagonist of formyl peptide receptor 2 (FPR2) signaling, which can inhibit WKYMVm binding to FPR2 with an C50 of 0.23 μM and can also inhibit intracellular calcium release induced by WKYMVm, MMK 1, amyloid β42, and F peptide. WRW4 inhibits FPR2-mediated signaling in human neutrophils and blocks chemotactic migration and superoxide generation by amyloid β42 peptide. WRW4 can reduce Brucella proliferation in animal models.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Bae et al (2004) Identification of peptides that antagonize formyl peptide receptor-like 1-mediating signaling. J.Immunol. 173 607 PMID: 15210823
• Kain et al (2018), Inhibition of FPR2 Impaired Leukocyte Get-in Signal and Triggers Non-Resolving Inflammation in Heart Failure. FASEB J. 32 287 PMID: 31216426
• Reyes et al (2021) Formyl peptide receptor 2 (FPR2) antagonism is a potential target for the prevention of Brucella abortus 544 infection, Immunobiology 226(3) 152073 https://doi.org/10.1016/j.imbio.2021.152073

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.