WLBU2 peptide

WLBU2 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RRWVRRVRRWVRRVVRVVRRWVRR

Arg-Arg-Trp-Val-Arg-Arg-Val-Arg-Arg-Trp-Val-Arg-Arg-Val-Val-Arg-Val-Val-Arg-Arg-Trp-Val-Arg-Arg

24

95.68%

3400.12

Synthetic

Powder

This peptide was active at physiologic NaCl concentrations against S. aureus and mucoid and nonmucoid strains of P. aeruginosa (see the ref). It also kills three oral microorganisms (grown planktonically): S. gordonii, F. nucleatum, and P. gingivalis (Antimicrob Agents Chemother. 2007 May;51(5):1837-9). WLBU2 also reduced the bacterial burden of S. gordonii and F. nucleatum biofilms. The minimal DNA bundling concentrations of LL-37 and WLBU2 are similar to the concentrations necessary to ensure effective bacteria killing. CSA-13, a member of the ceragenin family, like natural LL37 or synthetic WLBU2 and HB71 peptides targets the bacterial wall through interactions with LPS and LTA. The ability of CSA-13 to maintain its bactericidal activity in the presence of negatively charged F-actin and DNA and the observed additive effect of CSA-13 with rhDNase I suggest that this synthetic antibiotic has potential advantages in treating chronic infection in CF airways (J Antimicrob Chemother. 2007 Sep;60(3):535-45). Animal model:mouse: For the mice given peptide post-bacterial infection, in the 1 mg/kg group, nine of nine animals died because of Pseudomonas sepsis; in the 3 mg/kg group, only one of nine succumbed to infection and in the 4 mg/kg group, all mice were protected (P < 0.0001) by the peptide (Deslouches B et al., 2007). 10/30/2010. The source of WLBU2 is De novo peptide design using only residues V, R, and W.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Deslouches B, Phadke SM, Lazarevic V, et al. De novo generation of cationic antimicrobial peptides: influence of length and tryptophan substitution on antimicrobial activity. Antimicrob Agents Chemother. 2005;49(1):316-22.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.