WIKE-14 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

The WIKE-14 peptide is a synthetic α-helical antimicrobial peptide (AMP) with a helix-to-helix structure, derived from a series of designed XIW peptides. It features a substitution of glutamic acid at the 12th position, reducing its net positive charge while maintaining amphipathic properties. This peptide demonstrates strong binding affinity to lipopolysaccharides (LPS) via electrostatic interactions with phosphate groups and hydrophobic interactions with lipid A. In vitro studies reveal that WIKE-14 effectively suppresses LPS-induced pro-inflammatory cytokine production (e.g., TNF-α, IL-6) and reactive oxygen species (ROS) in macrophages by inhibiting LPS binding to cell surfaces. Its unique structural flexibility allows broad interaction with LPS molecules, contributing to potent anti-inflammatory effects.

Secondary structure analysis indicates that WIKE-14 adopts a dynamic helix-to-helix conformation in aqueous and LPS-bound states, distinguishing it from rigid α-helical counterparts. Unlike conventional AMPs, WIKE-14 exhibits negligible cytotoxicity at bioactive concentrations. In vivo, it mitigates LPS-induced inflammation in murine models, including ear edema and acute lung injury, without adverse effects. The peptide’s dual capacity for LPS neutralization and membrane interaction underscores its potential as a scaffold for developing multifunctional anti-inflammatory agents.

• Son H, Park SC, Kim YM, Lee JK, Park S, Guk T, Yoon AM, Lim HS, Jang MK, Lee JR. Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against Pseudomonas aeruginosa Endotoxin-Mediated Sepsis. Antibiotics (Basel). 2022 Nov 21;11(11):1675. doi: 10.3390/antibiotics11111675. PMID: 36421317; PMCID: PMC9686674.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.