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  • WIK-14 peptide

WIK-14 peptide

Not For Human Use, Lab Use Only.

Cat.#: 319844

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Product Information

  • Product Name
    WIK-14 peptide
  • Documents

    Batch to batch variation of the purity

  • Sequence Shortening
    H-WIKKIWKKIIKKIK-NH2
  • Sequence
    H-Trp-Ile-Lys-Lys-Ile-Trp-Lys-Lys-Ile-Ile-Lys-Lys-Ile-Lys-NH2
  • Length (aa)
    14
  • Peptide Purity (HPLC)
    96.19%
  • Molecular Formula
    C94H162N24O14
  • Molecular Weight
    1852.49
  • Source
    Synthetic
  • Form
    Powder
  • Description

    The WIK-14 peptide is a 14-amino acid α-helical antimicrobial peptide (AMP) designed with a sequence of WIKKIWKKIIKKIK, featuring lysine (K), isoleucine (I), and tryptophan (W) residues. Its amphipathic structure enables interactions with bacterial membranes through electrostatic and hydrophobic forces. Substitution of the 12th lysine with glutamic acid (E) yields WIKE-14 (WIKKIWKKIIKEIK), which adopts a helix-to-helix conformation. This structural flexibility enhances its binding capacity to lipopolysaccharides (LPS) via electrostatic interactions with phosphate groups and hydrophobic interactions with lipid A. CD spectroscopy confirms helical formation in LPS and membrane-mimetic environments. Both WIK-14 and WIKE-14 exhibit potent LPS-neutralizing activity, inhibiting pro-inflammatory cytokine release in macrophages by blocking LPS binding to TLR4/MD-2 receptors.

  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Son H, Park SC, Kim YM, Lee JK, Park S, Guk T, Yoon AM, Lim HS, Jang MK, Lee JR. Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against Pseudomonas aeruginosa Endotoxin-Mediated Sepsis. Antibiotics (Basel). 2022 Nov 21;11(11):1675. doi: 10.3390/antibiotics11111675. PMID: 36421317; PMCID: PMC9686674.
  • About TFA salt

    Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

    Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

    Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

    In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"