WH peptide

WH peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-WPRFHSSVFHTH-OH

H-Trp-Pro-Arg-Phe-His-Ser-Ser-Val-Phe-His-Thr-His-OH

12

95.48%

C₇₃H₉₆N₂₂O₁₆

1537.67

Synthetic

Powder

Peptide WH is a novel peptide discovered using phage display technique that selectively binds to Clec9a+ dendritic cells (DCs) or Clec9a over-expressed HEK-293T cells. It has been observed that Asp 248 and Trp 250 are two key residues for Clec9a to bind with peptide WH. When coupled with OVA 257-264 epitope, peptide WH can significantly enhance the ability of Clec9a+ DCs to activate OVA-specific CD8+ T cells, which elicit strong ability to secret IFN-γ, express perforin and granzyme B mRNA. Peptide WH could be considered as an antigen delivery carrier targeting Clec9a+ DCs for cancer immunotherapy. In brief, Peptide WH could bind to Clec9a+ DCs selectively, and enhance the antigen cross presentation, further stimulate the antigen specific CTL response both in vitro and in vivo. This strategy could be very effective in cancer immunotherapy.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Yan Z, Wu Y, Du J, Li G, Wang S, Cao W, Zhou X, Wu C, Zhang D, Jing X, Li Y, Wang H, Gao Y, Qi Y. A novel peptide targeting Clec9a on dendritic cell for cancer immunotherapy. Oncotarget. 2016 Jun 28;7(26):40437-40450. doi: 10.18632/oncotarget.9624. PMID: 27250027; PMCID: PMC5130018.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.