WH peptide

WH peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-WPRFHSSVFHTH-OH

H-Trp-Pro-Arg-Phe-His-Ser-Ser-Val-Phe-His-Thr-His-OH

12

95.48%

C₇₃H₉₆N₂₂O₁₆

1537.67

Synthetic

Powder

Peptide WH is a novel peptide discovered using phage display technique that selectively binds to Clec9a+ dendritic cells (DCs) or Clec9a over-expressed HEK-293T cells. It has been observed that Asp 248 and Trp 250 are two key residues for Clec9a to bind with peptide WH. When coupled with OVA 257-264 epitope, peptide WH can significantly enhance the ability of Clec9a+ DCs to activate OVA-specific CD8+ T cells, which elicit strong ability to secret IFN-γ, express perforin and granzyme B mRNA. Peptide WH could be considered as an antigen delivery carrier targeting Clec9a+ DCs for cancer immunotherapy. In brief, Peptide WH could bind to Clec9a+ DCs selectively, and enhance the antigen cross presentation, further stimulate the antigen specific CTL response both in vitro and in vivo. This strategy could be very effective in cancer immunotherapy.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Yan Z, Wu Y, Du J, Li G, Wang S, Cao W, Zhou X, Wu C, Zhang D, Jing X, Li Y, Wang H, Gao Y, Qi Y. A novel peptide targeting Clec9a on dendritic cell for cancer immunotherapy. Oncotarget. 2016 Jun 28;7(26):40437-40450. doi: 10.18632/oncotarget.9624. PMID: 27250027; PMCID: PMC5130018.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.