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Recombinant WEE1 protein (His tag)

Recombinant WEE1 protein (His tag)

Cat.#: 546905

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Special Price 294.7 USD

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Product Information

  • Product Name
    Recombinant WEE1 protein (His tag)
  • Documents
  • Description

    Recombinant Human WEE1hu (Wee1-like protein kinase) Fragment (292-646) is a high-purity protein encompassing the complete protein kinase domain (299-569). This fragment serves as an essential tool for investigating the regulation of cell cycle progression, specifically the G2 to M transition, by enabling targeted studies of kinase activity and CDK1 inhibition mechanisms.

    Wee1-like protein kinase (WEE1hu) is a key regulatory enzyme in human cells that acts as a negative regulator of mitotic entry by phosphorylating CDK1 on Tyr-15, thereby inactivating cyclin B1-CDK1 complexes and preventing premature transition from G2 to M phase. Its activity peaks during S and G2 phases and decreases during M phase, with protein levels declining at M/G1 phase likely due to degradation. The protein is localized to the nucleus, where it performs its critical function in maintaining proper cell cycle timing and genomic stability.

  • Protein name
    Wee1-like protein kinase
  • Uniprot ID
    P30291
  • Gene Name
    WEE1
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    DNA encoding 292-646 aa (P30291) were fused with 6His tag.
  • Protein Species
    Human
  • Molecular weight
    Predictes a molecular mass of 41.73 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 43 kDa band.
  • Purity
    >89%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"