VPM-p15 peptide

VPM-p15 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

VSXGILLDLSRTSLP, X is 4-methylphenylalanine

H-Val-Ser-Phe(4-Me)-Gly-Ile-Leu-Leu-Asp-Leu-Ser-Arg-Thr-Ser-Leu-Pro-OH

15

95.25%

C₇₅H₁₂₆N₁₈O₂₂

1631.91

Synthetic

Powder

VPM-p15 is an optimised version of the peptide p15, itself an activator derived from the ADGRG2 Stachel sequence, an agonist sequence found within within the large ectodomains of members of the adhesion G protein-coupled receptor (aGPCR) family. VPM-p15 activates ADGRG2 with significantly improved (>2 orders of magnitude) affinity compared to p15. VPM-p15 is a useful tool to investigate the function of ADGRG2, which is a critical regulator of male fertility that maintains ion/pH homeostasis and CFTR coupling.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Sun et al (2021) Optimization of a peptide ligand for the adhesion GPCR ADGRG2 provides a potent tool to explore receptor biology. J Biol Chem. 296 100174 PMID: 33303626

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.