VIRIP peptide

VIRIP peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-LEAIPMSIPPEVKFNKPFVF-OH

H-Leu-Glu-Ala-Ile-Pro-Met-Ser-Ile-Pro-Pro-Glu-Val-Lys-Phe-Asn-Lys-Pro-Phe-Val-Phe-OH

20

95.8%

C₁₁₂H₁₇₁N₂₃O₂₇S

2303.75

Synthetic

Powder

VIRIP is a natural subfragment of α1-antitrypsin (α1-AT) and is a broad-based inhibitor of HIV-1. VIRIP was identified from screening a human haemofiltrate derived library and corresponds to residues 353–37 of α1-AT, which is the most abundant circulating serine protease inhibitor. VIRIP directly targets the highly conserved viral gp41 fusion peptide to inhibit HIV-1 entry and inhibits a wide variety of HIV-1 strains including variants resistant to protease and reverse transcriptase inhibitors. VIRIP has little or no effect on infection by virions containing HIV-2, SIV, MLV, or VSV env proteins, or by other pathogens such as ebola, herpes simplex or respiratory syncytial virus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Münch et al (2007)Discovery and optimization of a natural HIV-1 entry inhibitor targeting the gp41 fusion peptide. Cell 129(2) 263 PMID: 17448989
• Müller et al (2018) Reduced Susceptibility to VIRIP-Based HIV-1 Entry Inhibitors Has a High Genetic Barrier and Severe Fitness Costs. J Virol. 92(17) e00733-18 PMID: 29925662

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.