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Product Name
Recombinant VATF protein (His tag)
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Description
Recombinant Human V-type proton ATPase subunit F (ATP6V1F) fragment (residues 1-119) is a high-quality, stable protein designed for structural and biochemical studies. This fragment represents a soluble and functional unit of the V-ATPase complex, ideal for investigating proton pump mechanisms and intracellular acidification processes.
V-type proton ATPase subunit F is a key component of the V1 complex in vacuolar (H+)-ATPase (V-ATPase), a multisubunit enzyme that hydrolyzes ATP to drive proton translocation across membranes. V-ATPase plays a critical role in acidifying intracellular compartments, such as cytoplasmic vesicles, secretory vesicles, synaptic vesicles, and clathrin-coated vesicles, and may also function at the plasma membrane to modulate extracellular pH. This enzyme is essential for maintaining pH homeostasis in cellular environments, impacting processes like protein sorting, neurotransmitter uptake, and organelle function. The recombinant fragment (residues 1-119) encompasses the full
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Protein name
V-type proton ATPase subunit F
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Uniprot ID
Q16864
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Gene Name
ATP6V1F; ATP6S14; VATF
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-119 aa (Q16864) were fused with C-terminal 6His tag.
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Sequence
MAGRGKLIAV IGDEDTVTGF LLGGIGELNK NRHPNFLVVE KDTTINEIED TFRQFLNRDD IGIILINQYI AEMVRHALDA HQQSIPAVLE IPSKEHPYDA AKDSILRRAR GMFTAEDLRL EHHHHHH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 14.42 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 16 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant VATF protein (His tag) SDS-PAGE
M: Marker; Lane 1: VATF; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"