Variegin [E31] peptide

Variegin [E31] peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SDQGDVAEPKMHKTAPPFDFEAIPEEYLDDES-OH

H-Ser-Asp-Gln-Gly-Asp-Val-Ala-Glu-Pro-Lys-Met-His-Lys-Thr-Ala-Pro-Pro-Phe-Asp-Phe-Glu-Ala-Ile-Pro-Glu-Glu-Tyr-Leu-Asp-Asp-Glu-Ser-OH

32

95.36%

C₁₅₈H₂₃₁N₃₇O₅₈S

3608.78

Synthetic

Powder

Variegin is a novel thrombin inhibitor found in the salivary gland extract of the tropical bont tick, Amblyomma variegatum. It is one of the smallest thrombin inhibitors known. Variegin binds to thrombin with strong affinity (K i ≈ 10.4 pM) and high specificity. The N-terminus of variegin affects the binding kinetics, with the removal of the seven residues leading to a change from fast to slow binding. The active site binding moiety of variegin is located in residues 8-14, while the exosite-I binding moiety is within residues 15-32. Variegin is structurally and functionally similar to the rationally designed thrombin inhibitor, hirulog, but it is a more potent inhibitor and retains its inhibitory activity even after cleavage by thrombin. Variegin provides a promising platform for the development of new thrombin inhibitors.
Variegin [E31] has the complete sequence of variegin without seven-residues removal on the N-terminal, so it is the version of Variegin with the fastest binding kinetic.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Koh CY, Kazimirova M, Trimnell A, et al. Variegin, a novel fast and tight binding thrombin inhibitor from the tropical bont tick. J Biol Chem. 2007;282(40):29101-13.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.