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Product Name
Human USP7/HAUSP (His & GST Tag) recombinant protein
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Protein name
Ubiquitin carboxyl-terminal hydrolase
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Protein short names
AA409944; AW548146; TEF1; HAUSP; AA617399; AU019296; USP7; C80752; 2210010O09RIK
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Uniprot ID
Q93009
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding full length of human USP7 (NP_003461.2) (Met 1-Asn 1102) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human USP7/GST chimera consists of 1339 amino acids and has a calculated molecular mass of 156 kDa. It migrates as an approximately 140 band in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA. Immobilized human P53 at 2 μg/ml (100 μl/well) can bind human USP7 with a linear ranger of 6.4-160 ng/ml.
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