-
Product Name
Recombinant UBC12 protein (His tag)
- Documents
-
Description
Recombinant Human UBE2M (Ubc12) Fragment (1-183) is a high-purity protein designed for studying NEDD8 conjugation mechanisms. This fragment fully encompasses the UBC core domain, making it an ideal tool for investigating E2 enzyme activity, protein neddylation, and related cellular processes in a controlled experimental setting.
The full-length NEDD8-conjugating enzyme Ubc12 (UBE2M) accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to target proteins. It specifically interacts with the E3 ubiquitin ligase RBX1 to neddylate cullin proteins such as CUL1, CUL2, CUL3, and CUL4, playing a critical role in regulating protein degradation pathways and cell proliferation. This enzyme is essential for understanding post-translational modification systems and their implications in cellular homeostasis and disease mechanisms.
-
Protein name
NEDD8-conjugating enzyme Ubc12
-
Uniprot ID
P61081
-
Gene Name
UBE2M; UBC12
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNA encoding 1-183 aa (P61081) were fused with 6His tag.
-
Protein Species
Human
-
Molecular weight
Predictes a molecular mass of 22.55 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 23 kDa band.
-
Purity
>88%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Activity
Not tested.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"