Transferrin Receptor Targeting Peptide T12 (TfR-T12)

Transferrin Receptor Targeting Peptide T12 (TfR-T12)

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-THRPPMWSPVWP-OH

H-Thr-His-Arg-Pro-Pro-Met-Trp-Ser-Pro-Val-Trp-Pro-OH

12

95.7%

C₇₁H₉₉N₁₉O₁₅S

1490.73

Synthetic

Powder

Transferrin receptor (TfR), is a transmembrane glycoprotein involved in cellular iron trafficking after interaction with its native iron-rich ligand, transferrin (Tf). Of the two TfR subtypes, theTfR2 is abundantly expressed in glioblastomas and their associated vasculature. Using the M13-phage screening on chicken embryo fibroblasts constitutively expressing the human transferrin receptor (hTfR), peptides T7 (HAIYPRH) and T12 (THRPPMWSPVWP) were discovered. Both peptides have been widely used to target several cancer types overexpressing transferrin receptors (TfR1 and TfR2), including liver, prostate, breast, pancreatic cancers, and many others. T12 showed higher affinity, in nanomolar range, towards the target and appeared more promising than the T7 peptide. However, T12 failed to show enhanced transport of conjugated compounds across the BBB.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wängler, C. et al. Mol Imaging Biol doi: 10.1007/s11307-010-0329-6, (2010).

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.