TIP peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

The TRPV1 inhibitory peptide (TIP) is a synthetic nine-amino acid peptide with the sequence Gln-Arg-Ala-Ile-Thr-Ile-Leu-Asp-Thr (QRAITILDT). It is designed to mimic a specific cytosolic region (residues 701–709) of the transient receptor potential vanilloid 1 (TRPV1) channel, which includes the critical phosphorylation site Thr705. TIP functions as a competitive inhibitor by binding to TRPV1 and preventing its phosphorylation and subsequent activation, thereby blocking calcium influx through the channel.

Studies demonstrate that TIP effectively inhibits TRPV1-mediated responses, including ultraviolet (UV)-induced upregulation of matrix metalloproteinases (MMPs) and pro-inflammatory cytokines. The peptide is cell-permeable and retains biological activity in epidermal keratinocytes and skin models, where it attenuates UV-induced molecular and cellular changes without acting as a physical UV filter.

• Kang SM, Han S, Oh JH, Lee YM, Park CH, Shin CY, Lee DH, Chung JH. A synthetic peptide blocking TRPV1 activation inhibits UV-induced skin responses. J Dermatol Sci. 2017 Oct;88(1):126-133. doi: 10.1016/j.jdermsci.2017.05.009. Epub 2017 May 17. PMID: 28551094.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.