Thymosin β4 (16-38) peptide

Thymosin β4 (16-38) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KLKKTETQEKNPLPSKETIEQEK

H-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-OH

23

97.2%

C₁₁₈H₂₀₄N₃₂O₄₁

2727.11

113318-05-1

Synthetic

Powder

Thymosin β4(16-38) is a peptide fragment. It belongs to the thymosin family. This peptide has been shown to be an activator of calmodulin-dependent enzymes. It can enhance the phosphorylation of myosin light chains (LC2​) in the absence of exogenous CaM, and it is a Ca²⁺-independent high-affinity stimulator of myosin light chain kinase (MLCK). In experiments comparing its stimulatory effect on MLCK basal activity with other substances like C3​ and thymosin α1​ , it was found that all these compounds can activate the enzyme without relying on Ca²⁺ , and C3​ > Tβ4​ (16−38) > (CaM+Ca²⁺)>α1​ in terms of the potency of activating the enzyme.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• T.Abiko and H.Sekino, Chem. Pharm. Bull., 35, 3757 (1987).
• Galoyan AA, Gurvitis BYa, Shuvalova LA, Davis MT, Shively JE, Lee TD. A hypothalamic activator of calmodulin-dependent enzymes is thymosin beta 4 (1-39). Neurochem Res. 1992 Aug;17(8):773-7. doi: 10.1007/BF00969011. PMID: 1641059.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.