Thanatin peptide

Thanatin peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GSKKPVPIIYCNRRTGKCQRM

H-Gly-Ser-Lys-Lys-Pro-Val-Pro-Ile-Ile-Tyr-Cys-Asn-Arg-Arg-Thr-Gly-Lys-Cys-Gln-Arg-Met-OH (disulfide bond: Cys¹¹-Cys¹⁸)

21

98.2%

C₁₀₃H₁₇₇N₃₅O₂₇S₃

2433.96

16143622

Synthetic

Powder

Thanatin is an inducible 21-residue cationic antimicrobial peptide that was first isolated from the hemolymph of the hemipteran insect Podisus maculiventris (spined soldier bug), through immune challenge. Thanatin is strongly cationic (pI of 10.48) and contains a distinct short eight-residue basic loop created through a disulfide bond formation between residues Cys11 and Cys18 at the C-terminus. Thanatin exerts bactericidal activity by disrupting outer membrane(OM) integrity. Thanatin damages the OM by promoting the release of divalent cations. Thanatin competitively replaces the divalent cations to bind to LPS, thereby disrupting the integrity of OM and leading to bacterial death.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ma, B., Fang, C., Lu, L. et al. The antimicrobial peptide thanatin disrupts the bacterial outer membrane and inactivates the NDM-1 metallo-β-lactamase. Nat Commun 10, 3517 (2019).
• Dash R, Bhattacharjya S. Thanatin: An Emerging Host Defense Antimicrobial Peptide with Multiple Modes of Action. Int J Mol Sci. 2021 Feb 3;22(4):1522. doi: 10.3390/ijms22041522. PMID: 33546369; PMCID: PMC7913509.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.