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Recombinant TFB2M protein (His tag)

Cat.#: 541259

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Product Information

  • Product Name
    Recombinant TFB2M protein (His tag)
  • Documents
  • Description

    Recombinant human Dimethyladenosine transferase 2 (TFB2M) fragment (residues 72-396), a stable and soluble unit ideal for investigating mitochondrial transcription initiation and rRNA methylation mechanisms in structural and biochemical studies.

    Dimethyladenosine transferase 2 (TFB2M) is a mitochondrial protein that functions as an S-adenosyl-L-methionine-dependent rRNA methyltransferase, likely methylating specific adenosines in 12S mitochondrial rRNA. It is a key component of the mitochondrial transcription initiation complex, which includes TFAM and POLRMT, and is essential for basal transcription of mitochondrial DNA. TFB2M facilitates promoter opening and structural changes in POLRMT, independent of its methyltransferase activity, highlighting its dual role in mitochondrial gene expression. The protein is localized exclusively in the mitochondrion, underscoring its importance in mitochondrial function and energy metabolism.

  • Protein name
    Dimethyladenosine transferase 2, mitochondrial
  • Uniprot ID
    Q9H5Q4
  • Gene Name
    TFB2M; NS5ATP5
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    DNA encoding 72-396 aa (Q9H5Q4) were fused with C-terminal 6His tag.
  • Sequence 
    MSKIKKRYVT DRRLAETLAQ IYLGKPSRPP HLLLECNPGP GILTQALLEA GAKVVALESD KTFIPHLESL GKNLDGKLRV
IHCDFFKLDP RSGGVIKPPA MSSRGLFKNL GIEAVPWTAD IPLKVVGMFP SRGEKRALWK LAYDLYSCTS IYKFGRIEVN
MFIGEKEFQK LMADPGNPDL YHVLSVIWQL ACEIKVLHME PWSSFDIYTR KGPLENPKRR ELLDQLQQKL YLIQMIPRQN
LFTKNLTPMN YNIFFHLLKH CFGRRSATVI DHLRSLTPLD ARDILMQIGK QEDEKVVNMH PQDFKTLFET IERSKDCAYK
WLYDETLEDR LEHHHHHH
  • Protein Species
    Human
  • Molecular weight
    Predictes a molecular mass of 39.19 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 39 kDa band.
  • Purity
    >90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.
  • Validations

    Recombinant TFB2M protein (His tag) SDS-PAGE

    Recombinant TFB2M protein (His tag) SDS-PAGE

    M: Marker; Lane 1: TFB2M

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"