Temporin-SHa peptide

Temporin-SHa peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FLSGIVGMLGKLF-NH2

Phe-Leu-Ser-Gly-Ile-Val-Gly-Met-Leu-Gly-Lys-Leu-Phe-NH2

13

97.39%

C₆₇H₁₀₉N₁₅O₁₄S

1380.73

Synthetic

Powder

C-terminal NH2. Active against S. aureus ATCC 25923 (MIC 3 uM), E. faecalis ATCC 29212 (MIC 10 uM), B. megaterium (2 uM), Gram-negative E. coli ATCC 25922 (MIC 10 uM), E. coli ATCC 35218 (MIC 10 uM), P. aeruginosa ATCC 27853 (MIC 20 uM), fungi C. albicans ATCC 90028 (MIC 16 uM), C. parapsilosis ATCC 22019 (MIC 31 uM), and S. cerevisiae (MIC 8 uM). 50% hemolysis at 25 uM. The peptide is helical in 40% TFE based on CD. 3D structure bound to SDS micelles is known with the three glycines on the polar surface (Biochemistry 2008; 47: 10513-25). Updated 3/2014. Temporin-SHa has Antibacterial, Antifungal activity. The source of Temporin-SHa is the skin of the Sahara frog Pelophylax (Rana) saharica originating from Tunisia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Abbassi F, Oury B, Blasco T, et al. Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica. Peptides. 2008;29(9):1526-33.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.