Temporin-PTa peptide

Temporin-PTa peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FFGSVLKLIPKIL

Phe-Phe-Gly-Ser-Val-Leu-Lys-Leu-Ile-Pro-Lys-Ile-Leu

13

96.8%

Synthetic

Powder

Activity of the natural form can't be evaluated accurately due to low amount of the peptide. A synthetic peptide was found to be active against E. coli and S. aureus USA300 (MRSA) (Mishra B and Wang G 2012 JACS). The L and D-forms of the peptide showed identical antibacterial activity against E. coli (MIC=25 uM),B. subtilis (MIC=6.25 uM), and S. aureus (MIC=3.1 uM) (D=L). 3D structure to be published (G Wang). In addition, a mutant was found to be HIV active (EC50=0.63 uM) (Wang G et al. 2010 Antimicrob. Agents Chemother. 54: 1343-1346). The same mutant also displayed in vivo efficacy in preventing biofilm formation in an animal model:mouse (Menousek J et al 2012). APD update 7/19/2012. Temporin-PTa has Antibacterial activity. The source of Temporin-PTa is Hylarana picturata, Asia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Conlon JM, Kolodziejek J, Nowotny N, et al. Characterization of antimicrobial peptides from the skin secretions of the Malaysian frogs, Odorrana hosii and Hylarana picturata (Anura:Ranidae). Toxicon. 2008;52(3):465-73.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.