Temporin [K3]SHa peptide

Temporin [K3]SHa peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FLKGIVGMLGKLF-NH2

H-Phe-Leu-Lys-Gly-Ile-Val-Gly-Met-Leu-Gly-Lys-Leu-Phe-NH2

13

95.41%

C₇₀H₁₁₆N₁₆O₁₃S

1421.83

Synthetic

Powder

Temporin [K3]SHa is a synthetic peptide analog of temporin-SHa (SHa). It was designed to improve the antibacterial and antiparasitic activity of SHa. [K3]SHa exhibits potent activity against a wide range of bacteria (MIC = 1 - 6 μM for most tested bacteria), yeasts/fungi (MIC = 3 - 25 μM), and trypanosomatids (Leishmania and Trypanosoma). It is also effective against antibiotic-resistant strains of S. aureus and antimony-resistant L. infantum. [K3]SHa is significantly more active than the parent peptide SHa, especially against Gram-negative bacteria and yeasts/fungi. For example, the MIC values decreased by approximately 8-fold for P. aeruginosa, 4-fold for S. enterica, and 3-fold for E. coli. [K3]SHa also has potent and rapid antiprotozoal activities against different species of Leishmania (responsible for visceral, cutaneous, and mucocutaneous leishmaniases) and Trypanosoma (T. brucei gambiense and T. cruzi).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Raja Z, André S, Abbassi F, Humblot V, Lequin O, Bouceba T, Correia I, Casale S, Foulon T, Sereno D, Oury B, Ladram A. Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent. PLoS One. 2017 Mar 20;12(3):e0174024. doi: 10.1371/journal.pone.0174024. PMID: 28319176; PMCID: PMC5358776.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.