Temporin-1RNb peptide

Temporin-1RNb peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FLPLKKLRFGLL-NH2

H-Phe-Leu-Pro-Leu-Lys-Lys-Leu-Arg-Phe-Gly-Leu-Leu-NH2

12

95.13%

C₇₃H₁₂₂N₁₈O₁₂

1443.86

Synthetic

Powder

Temporin-1RNb is an antimicrobial peptide isolated from the skin secretions of the black-spotted frog, Rana nigromaculata, Northeastern China, Asia.
Temporin-1RNb belongs to the temporin family of antimicrobial peptides. Temporin-1RNb exhibits strong antimicrobial activity against both Gram-positive and Gram-negative bacteria, as well as the yeast pathogen Candida albicans. Specifically, it is active against E. coli, P. aeruginosa (MIC 12.5 uM), S. aureus, B. cereus, S. lactis (MIC 3.13 uM), C. albicans (MIC 6.25 uM), and cancer cells MCF 7 cells and Hela cells (IC50 ~12 uM). HC50 (50% hemolysis at 136 uM). Temporin-1RNb has a higher antimicrobial potency compared to other temporin peptides, likely due to specific amino acid substitutions.
Temporin-1RNb adopts an extended alpha-helical conformation in a membrane-mimetic environment, indicating its potential to interact with anionic membranes. Temporin-1RNb does not show haemolytic activity against human erythrocytes or toxicity against tested cancer cell lines, suggesting its selective cytotoxic activity against bacteria and cancer cells with negatively charged membranes.
Temporin-1RNb is helical in SDS or TFE.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Li A, Zhang Y, Wang C, Wu G, Wang Z. Purification, molecular cloning, and antimicrobial activity of peptides from the skin secretion of the black-spotted frog, Rana nigromaculata. World J Microbiol Biotechnol. 2013;29(10):1941-9.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.