Teduglutide peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Teduglutide is a synthetic analog of glucagon-like peptide-2 (GLP-2), a naturally occurring peptide hormone involved in intestinal growth and function. Structurally, teduglutide differs from native GLP-2 by a single amino acid substitution, which confers resistance to degradation by dipeptidyl peptidase IV (DPP-IV), thereby extending its half-life. This modification enhances its biological activity in promoting intestinal mucosal growth. Studies in animal models demonstrate that teduglutide increases villus height, crypt depth, and epithelial cell proliferation in the small intestine and colon. Additionally, it enhances nutrient transport capacity, particularly for glucose and glutamine, across the intestinal epithelium.

The peptide exerts its effects by binding to the GLP-2 receptor, which is expressed in the gastrointestinal tract. Activation of this receptor signaling pathway stimulates cellular proliferation and inhibits apoptosis, leading to increased mucosal surface area. Teduglutide has been shown to modulate intestinal adaptation following resection, with effects observed in both structural and functional parameters. Its mechanisms include upregulation of absorptive surface area and transient enhancement of nutrient processing capacity in the intestine.

• Naberhuis JK, Deutsch AS, Tappenden KA. Teduglutide-Stimulated Intestinal Adaptation Is Complemented and Synergistically Enhanced by Partial Enteral Nutrition in a Neonatal Piglet Model of Short Bowel Syndrome. JPEN J Parenter Enteral Nutr. 2017 Jul;41(5):853-865. doi: 10.1177/0148607115602891. Epub 2015 Aug 24. PMID: 26304601.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.