Tat-NTS peptide

Tat-NTS peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-YGRKKRRQRRRRSFPHLRRVF-NH2

H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Arg-Ser-Phe-Pro-His-Leu-Arg-Arg-Val-Phe-NH2

21

95.87%

C₁₂₅H₂₁₂N₅₄O₂₄

2855.36

Synthetic

Powder

Tat-NTS peptide is a cell-penetrating peptide consisting of Tat fused to amino acid residues R228 to F237 of the repeat III domain of ANXA1, which functions as a unique nuclear translocation signal (NTS). Tat-NTS peptide specifically blocks the interaction of ANXA1 with importin β and consequently the nuclear translocation of ANXA1. Tat-NTS peptide inhibits the transcriptional activity of p53, decreases Bid expression, suppresses activation of the caspase-3 apoptosis pathway and improves survival of neurons subjected to oxygen-glucose deprivation in vitro. In a focal brain ischemia animal model, Tat-NTS peptide reduces neuronal apoptosis in the ischemic zone and effects a dramatic reduction of infarct volume and a parallel improvement in neurological function after reperfusion.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Li et al (2019) A novel cell-penetrating peptide protects against neuron apoptosis after cerebral ischemia by inhibiting the nuclear translocation of annexin A1. Cell Death Differ. 26(2) 260 PMID: 29769639

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.