Tat-GluA2-3Y peptide

Tat-GluA2-3Y peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-YGRKKRRQRRRYKEGYNVYG-OH

H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Tyr-Lys-Glu-Gly-Tyr-Asn-Val-Tyr-Gly-OH

20

97.9%

C₁₁₅H₁₈₅N₄₃O₂₉

2633.98

1404188-93-7

Synthetic

Powder

The synthetic peptide (Tat-Glur23Y/Tat-GluA2-3Y/Tat-GluA2(3Y)) contains tyrosine residues that blocks phosphorylation of alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor endocytosis. Previous research shows that Tat-Glur23Y blocks regulated AMPA and thereby prevents long-term depression (LTD) in structures such as the nucleus accumbens and dorsal hippocampus. Tat-GluA2-3Y peptide could competitively inhibits binding of Syt3 to a tyrosine-rich (3Y) motif on the cytoplasmic tail of GluA2. Injecting the Tat-GluA2-3Y peptide in wild-type mice mimics the lack of forgetting of spatial memories, and this effect is occluded in Syt3 knockout mice.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Awasthi A, Ramachandran B, Ahmed S, et al. Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting. Science. 2019;363(6422)

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.