TAT-DEF-Elk-1 peptide
Not For Human Use, Lab Use Only.
Cat.#: 319834
Special Price 349.00 USD
Availability:
4 weeks
-
Product Name
TAT-DEF-Elk-1 peptide
-
Documents
Batch to batch variation of the purity
-
Sequence Shortening
H-GRKKRRQRRRPPSPAKLSFQFPSSGSAQVHI-OH
-
Sequence
H-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Ser-Pro-Ala-Lys-Leu-Ser-Phe-Gln-Phe-Pro-Ser-Ser-Gly-Ser-Ala-Gln-Val-His-Ile-OH
-
Length (aa)
31
-
Peptide Purity (HPLC)
95.44%
-
Molecular Formula
C155H259N57O40
-
Molecular Weight
3561.06
-
Source
Synthetic
-
Form
Powder
-
Description
The TAT-DEF-Elk-1 peptide is a synthetic cell-penetrating peptide designed to specifically interfere with the DEF docking domain of the transcription factor Elk-1. This domain is crucial for the interaction between Elk-1 and activated extracellular signal-regulated kinases (ERKs). The peptide mimics the DEF domain, thereby blocking ERK-mediated phosphorylation of Elk-1 on critical serine residues (Ser383/389) without affecting ERK activation or its ability to phosphorylate other substrates such as MSK-1 and CREB.
Elk-1 is a key transcription factor involved in neuronal differentiation and cytoskeleton dynamics. Its phosphorylation by ERKs triggers nuclear translocation and subsequent serum response element (SRE)-dependent gene expression. The TAT-DEF-Elk-1 peptide effectively prevents Elk-1 nuclear translocation and phosphorylation, providing a tool to study the specific role of Elk-1 activation in cellular processes. This peptide has been shown to differentially regulate immediate early gene expression compared to classical ERK pathway inhibitors, highlighting its specificity for Elk-1-dependent pathways.
-
Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
-
References
- Lavaur J, Bernard F, Trifilieff P, Pascoli V, Kappes V, Pagès C, Vanhoutte P, Caboche J. A TAT-DEF-Elk-1 peptide regulates the cytonuclear trafficking of Elk-1 and controls cytoskeleton dynamics. J Neurosci. 2007 Dec 26;27(52):14448-58. doi: 10.1523/JNEUROSCI.2279-07.2007. PMID: 18160653; PMCID: PMC6673434.
-
About TFA salt
Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.
Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.
Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.
In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.
-
Molar Concentration Calculator
-
Dilution Calculator
-
Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"