TAT-CBD3 peptide

TAT-CBD3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-YGRKKRRQRRRARSRLAELRGVPRGL-OH

H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Ala-Arg-Ser-Arg-Leu-Ala-Glu-Leu-Arg-Gly-Val-Pro-Arg-Gly-Leu-OH

26

95.19%

C₁₃₄H₂₄₃N₅₉O₃₂

3192.73

Synthetic

Powder

TAT-CBD3 is the 15-amino acid peptide derived from the axonal collapsin response mediator protein 2 (CRMP-2) conjugated to the cell penetrating motif of the HIV transduction domain protein TAT, which is a modulator of N-type voltage-gated calcium channels (CaV2.2). TAT-CBD3 functions as a CRMP2 - CaV2.2 interaction inhibitor and reduces CaV2.2 mediated currents, and also reduces T-type currents, which results in reduced nociceptor excitability, diminished neuropeptide release from sensory neurons and lower excitatory synaptic transmission. TAT-CBD3 can also reduce hypersensitive behaviour in chemical-induced inflammatory and neuropathic animal pain models.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Brittain et al (2011) Suppression of inflammatory and neuropathic pain by uncoupling CRMP-2 from the presynaptic Ca2+ channel complex. Nature Med. 17(7) 822 PMID: 21642979
• Fischer et al (2014) Sustained relief of neuropathic pain by AAV-targeted expression of CBD3 peptide in rat dorsal root ganglion. Gene Ther. 21(1) 44 PMID: 24152582

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.