Tat (48-60) peptide

Tat (48-60) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GRKKRRQRRRPPQ-OH

H-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-OH

13

95.65%

C₇₀H₁₃₁N₃₅O₁₆

1719.01

Synthetic

Powder

Tat (48 - 60), also known as HIV-1 Tat (48 - 60), is a cell penetrating cationic peptide derived from the N-terminus of the Tat protein, which is a transactivator of the transcription protein present in the human immunodeficiency virus (HIV). Tat (48-60) is an arginine-rich basic peptide which has three domains that function to aid HIV through transactivation, DNA binding and nuclear transport. Tat (48-60) posseses antibacterial activity but is mainly used for the non disruptive transport of exogenous macromolecules into cells.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Futaki (2005) Membrane-permeable arginine-rich peptides and the translocation mechanisms. Adv. Drug Deliv Rev. 57(4) 547 PMID: 15722163
• Dennison et al (2007) Interactions of cell penetrating peptide Tat with model membranes: A biophysical study. Biochem. Biophys. Res. Commun. 363(1) 178 PMID: 17854767
• Zhu and Shin (2009) Effects of dimerization of the cell-penetrating peptide Tat analog on antimicrobial activity and mechanism of bactericidal action. J. Pept. Sci. 2009 15(5) 345 PMID: 19206074

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.