Taromycin A peptide

Taromycin A peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

WNDTGKDADGAEY

Trp-Asn-Asp-Thr-Gly-Lys-Asp-Ala-Asp-Gly-Ala-Glu-Tyr

13

95.9%

Synthetic

Powder

~82% similar to AP2203: daptomycin. The authors used a transformation-associated recombination (TAR)-based genetic platform to directly clone, refactor, and heterologously express a silent biosynthetic pathway to yield a new antibiotic analog of daptomycin. The marine actinomycete Saccharomonospora sp. CNQ-490 TAR gene cluster was successfully expressed. Taromycin A differs from Daptomycin only in serveal places: 1) residue T11 is an D-Ala; 2) W is chlorinated at position 6; 3) Kny is also chlorinated at position 4; and 4) it carried an octa-2,4-dienoyl lipid side chain. Warning: the sequence is a close mimic of the natural counterpart. For example, kynurenine-13 (Kyn) is represented by Y here. Refer to daptomycin for more details. Active against MRSA 0325 (MIC 12 ug/ml), E. faecalis 613, E. faecalis 447 (MIC 6 ug/ml), E. faecalis 613D, E. faecium 447D (MIC 50 ug/ml), and VRE 5938 (100 ug/ml). The source of Taromycin A is the marine actinomycete Saccharomonospora sp. CNQ-490.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Yamanaka K, Reynolds KA, Kersten RD, et al. Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc Natl Acad Sci USA. 2014;111(5):1957-62.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.